Andrew Bartko, Battelle Memorial Institute, discussed the use of a novel Raman spectroscopy technology for the rapid identification of microbial contaminants in clean room environments. He provided an overview of how EM samples can be introduced into the Raman system, where each particle or organism cell can be detected, enumerated and identified. Low level detection studies were also discussed, where it was demonstrated that Raman can count and identify multiple types of microorganisms, such as E. coli and C. albicans, in the same sample. The technology is non-destructive, allowing for additional confirmatory analysis if so desired, and can also be adapted to perform microbial identifications on cells that have been stained with a viability substrate, which is what we would normally use with flow cytometry and solid phase cytometry RMMs
Andrew Bartko, Battelle Memorial Institute, discussed the use of a novel Raman spectroscopy technology for the rapid identification of microbial contaminants in clean room environments. He provided an overview of how EM samples can be introduced into the Raman system, where each particle or organism cell can be detected, enumerated and identified. Low level detection studies were also discussed, where it was demonstrated that Raman can count and identify multiple types of microorganisms, such as E. coli and C. albicans, in the same sample. The technology is non-destructive, allowing for additional confirmatory analysis if so desired, and can also be adapted to perform microbial identifications on cells that have been stained with a viability substrate, which is what we would normally use with flow cytometry and solid phase cytometry RMMs